Hello and welcome to my second blog for PhD trip!
This time I would like to talk in more detail about my research. I work in different fields starting from polymer chemistry to biochemistry and biological interactions. My task is to create a microsieve-based biosensor for analysis of food.
A surface that interacts with a biological medium is called a bioactive surface. The surface is a focal point in the design of any biosensor. Thus, the first task for me to tackle in the design of my sensor. A bioactive surface consist of three main components: a surface of course, an antifouling layer and, a bioactive element. In my case we use as surface a microsieve, in simple words this is like a microscopic version of a sieve. The microsieve has a thin silicon nitride membrane layer (~1 µm) that has small uniform holes in it with size in the range from 0.45 to 5 micrometers.
Second is the antifouling layer. So, what is an antifouling surface? It is a surface that resist adsorption of proteins form biological liquids. Those undesirable proteins create problems for functioning of biosensor. The creation of a surface that will resist to non-specific attachment of proteins is a challenging but incredibly fascinating task. Furthermore, this surface will be functionalized with antibodies or any other receptor. These bioactive surfaces will become the heart of future biosensors.
This is shortly about my research work!
Thanks till next blog!